China Supplier high quality Pyruvate Oxidase CAS 9001-96-1 CAS NO.9001-96-1

Professional supplier Pyruvate Oxidase CAS 9001-96-1 with superior quality

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Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical & its intermediates, Food/Feed additives, Fine chemicals in distributing the products of our own company and affiliated enterprises, are the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients’ requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves. 

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.

We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward powder, their satisfaction is our struggling goal.

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Pyruvate oxidase is an enzyme found in certain bacteria, such as Escherichia coli, that plays a role in aerobic carbohydrate metabolism. Here's a detailed breakdown of its function and characteristics:

Reaction Catalyzed

Pyruvate oxidase catalyzes the oxidative decarboxylation of pyruvate, converting it into acetyl phosphate, CO?, and hydrogen peroxide (H?O?). The reaction is:

Pyruvate+Phosphate (Pi)+O2→Acetyl Phosphate+CO2+H2O2Pyruvate+Phosphate (Pi)+O2?→Acetyl Phosphate+CO2?+H2?O2?

Acetyl phosphate serves as a high-energy intermediate that can donate a phosphate group to ADP, generating ATP via substrate-level phosphorylation.

H?O? is a reactive byproduct, which bacteria neutralize using enzymes like catalase.

Cofactors

Thiamine pyrophosphate (TPP): Facilitates decarboxylation of pyruvate.

Flavin adenine dinucleotide (FAD): Acts as an electron carrier, transferring electrons to oxygen (the final acceptor).

Biological Role

1. Energy Production: Generates ATP directly via acetyl phosphate, bypassing the citric acid cycle and electron transport chain. This is advantageous under specific conditions (e.g., high oxygen, low glucose).

2. Acetate Metabolism: Acetyl phosphate can be converted to acetate, a key metabolic intermediate in bacteria.

3. Metabolic Flexibility: Provides an alternative pathway when pyruvate dehydrogenase (PDH) is inactive or insufficient, enhancing survival in varying environments.

Regulation

Activity is influenced by oxygen availability, substrate levels, and metabolic demands. For example, in E. coli, the enzyme is encoded by the poxB gene and is upregulated under aerobic conditions.

Organisms and Localization

Primarily found in prokaryotes (e.g., E. coli, Lactobacillus). The enzyme operates in the cytoplasm, as bacteria lack mitochondria.

Comparison with Pyruvate Dehydrogenase (PDH)

PDH converts pyruvate to acetyl-CoA (for the citric acid cycle) and relies on NAD?, producing CO? and NADH. It is part of oxidative phosphorylation, yielding more ATP.

Pyruvate oxidase is less energy-efficient (1 ATP vs. ~10–12 ATP via PDH/ETC) but offers rapid ATP production and metabolic versatility.

Key Takeaways

Function: Links glycolysis to acetate metabolism and substrate-level phosphorylation in bacteria.

Adaptive Role: Helps bacteria thrive in aerobic environments by balancing energy production and oxidative stress management.

Biotechnological Relevance: Understanding this enzyme aids in metabolic engineering and antibiotic development targeting bacterial pathways.

In summary, pyruvate oxidase exemplifies bacterial metabolic ingenuity, enabling energy production and adaptability through alternative biochemical routes.