Nitrotetrazolium Blue Chloride NBT CAS 298-83-9 Use for Enzyme Activity Assays CAS NO.298-83-9

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Description of Nitrotetrazolium Blue Chloride from Wuhan Fortuna Chemical Co.,Ltd

Basic Information of Nitrotetrazolium Blue Chloride
Product Name	Nitrotetrazolium Blue Chloride
CAS NO.	298-83-9
Apperance	Yellow Powder
Assay	98%
Pacakge	100G/500G/1KG/Bag
MOQ	1kg
Storage	Cool and dry place
Shelf Life	2 Years
Brand	Watson

Nitrotetrazolium Blue Chloride NBT CAS 298-83-9 Use for Enzyme Activity Assays

 

Nitrotetrazolium Blue Chloride (NBT) is a yellow, water-soluble tetrazolium salt that is widely used in biochemical and histochemical applications. Its primary function is as an electron acceptor in various assays, where it is reduced to an insoluble, deeply colored formazan product (blue-purple). Here are its key applications:

 

 1. Enzyme Activity Assays

   - Superoxide Detection (NBT Reduction Test)

     NBT is commonly used to detect superoxide radicals (O??) produced by enzymes like NADPH oxidase (e.g., in phagocytic cells) or xanthine oxidase. When reduced by superoxide, it forms formazan deposits, which can be quantified spectrophotometrically.

   - Dehydrogenase Activity

     NBT serves as an electron acceptor in assays measuring dehydrogenase enzymes (e.g., in mitochondrial respiration studies).

 

 2. Microbiology & Cell Biology

   - NBT Test for Bacterial Respiration

     Used to differentiate bacteria based on their ability to reduce NBT (e.g., in oxidative stress assays).

   - Phagocytosis & Respiratory Burst Assays

     In immunology, NBT is used to measure the respiratory burst activity of neutrophils and macrophages, indicating their antimicrobial function.

 

 3. Histochemistry & Microscopy

   - Localization of Reactive Oxygen Species (ROS)

     NBT is used in tissue sections or whole mounts (e.g., plant tissues) to visualize sites of ROS production, such as in plant stress responses or inflammatory conditions.

   - Alkaline Phosphatase (ALP) Staining

     In combination with other reagents, NBT can help visualize ALP activity in cells or tissues.

 

 4. Plant Science

   - Detection of Oxidative Stress in Plants

     NBT staining is employed to study superoxide production during abiotic stress (e.g., drought, salinity) or pathogen attacks.

 

 5. Clinical Diagnostics

   - Chronic Granulomatous Disease (CGD) Screening

     The NBT slide test diagnoses CGD, a genetic disorder where phagocytes cannot produce superoxide radicals, leading to impaired NBT reduction.

 

Key Advantages of NBT:

   - Forms insoluble formazan, allowing precise localization. 

   - Sensitive to superoxide and other reductants. 

   - Compatible with spectrophotometric and microscopic analysis. 

 

Limitations:

   - Can be reduced by non-specific reductants, requiring controlled conditions. 

   - Formazan deposits may interfere with some staining techniques. 

 

FAQ From Wuhan Fortuna Chemical Co.,Ltd
Q	Can I get a sample for free ?
A	For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test.
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A	Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order.
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A	For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement.
Q	How long is your after-sales service?
A	We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company.
Q	How do you treat quality complaint ?
A	We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers.

Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide Nitrotetrazolium Blue Chloride with good quality and best price, welcome to contact us to get COA/TDS/MSDS of Nitrotetrazolium Blue Chloride.