China manufacturer Supply high quality DEAE-Dextran CAS 9015-73-0 with good price CAS NO.9015-73-0

Professional supplier DEAE-Dextran CAS 9015-73-0 with superior quality

Company profile

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical & its intermediates, Food/Feed additives, Fine chemicals in distributing the products of our own company and affiliated enterprises, are the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients’ requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves. 

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.

We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward powder, their satisfaction is our struggling goal.

our competitive advantage

Guaranteed purity

High quality & competitive price

Quality control

Fast feedback

Prompt shipment

Description of DEAE-Dextran from Wuhan Fortuna Chemical Co.,Ltd

Specifications(COA) of DEAE-Dextran CAS 9015-73-0

Introduction of DEAE-Dextran is a cationic polymer derived from dextran (a glucose polysaccharide) modified with diethylaminoethyl (DEAE) groups, giving it a positive charge at neutral pH. Primarily used in molecular biology, it facilitates transfection by binding negatively charged DNA, forming complexes that adhere to cell membranes for uptake. It also serves as an anion exchanger in chromatography (e.g., DEAE-Sephadex) to purify proteins/nucleic acids. While largely replaced by lipofection or electroporation due to cytotoxicity at high doses, it remains cost-effective for specific cell types. Additional roles include nucleic acid precipitation and experimental drug/gene delivery. Optimal use requires pH control and minimized exposure to reduce cell damage.

Application of DEAE-Dextran is a chemically modified polysaccharide used primarily in molecular biology and biochemistry. Here's a structured overview:

Chemical Composition

Dextran Base: A polysaccharide composed of glucose units, known for its solubility and biocompatibility.

DEAE Modification: Diethylaminoethyl groups are attached, conferring a positive charge at physiological pH, making it a cationic polymer.

Key Applications

1. Transfection

Mechanism: Binds negatively charged DNA via electrostatic interactions, forming complexes that adhere to cell membranes. This facilitates cellular uptake, potentially through endocytosis or membrane disruption.

Use Case: Historically employed for introducing DNA into mammalian cells (e.g., COS, HeLa). Efficiency varies by cell type and requires optimization to balance efficacy and cytotoxicity.

Current Status: Largely supplanted by lipofection and electroporation but remains cost-effective for specific applications.

2. Chromatography

Ion Exchange Resin: DEAE-Dextran (e.g., DEAE-Sephadex) acts as a weak anion exchanger, separating proteins or nucleic acids based on charge. Used in purifying negatively charged biomolecules.

3. Other Uses

Nucleic Acid Precipitation: Can aggregate DNA/RNA via charge neutralization.

Drug/Gene Delivery: Explored as a carrier for charged therapeutic molecules, though toxicity limits in vivo use.

Considerations

Cytotoxicity: High concentrations can damage cells, restricting exposure time in transfection protocols.

pH Sensitivity: Charge density depends on pH, requiring buffered conditions for optimal performance.

Conclusion

DEAE-Dextran is a versatile tool in biotechnology, leveraging its cationic properties for transfection and chromatography. While newer methods have reduced its prevalence, it remains valuable in niche applications where cost and simplicity are prioritized.

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is  the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.

Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide DEAE-Dextran with good quality and best price, welcome to contact us to get COA/TDS/MSDS of DEAE-Dextran.