China Suppliers high quality 3,3'-Diaminobenzidine CAS 91-95-2 with good price CAS NO.91-95-2

Professional supplier 3,3'-Diaminobenzidine CAS 91-95-2 with superior quality

Company profile

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical & its intermediates, Food/Feed additives, Fine chemicals in distributing the products of our own company and affiliated enterprises, are the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients’ requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves. 

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.

We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward powder, their satisfaction is our struggling goal.

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3,3'-Diaminobenzidine (DAB) is a chemical compound primarily used in biochemical and histological applications. Here's a detailed overview:

Chemical Structure

Structure: DAB consists of two benzene rings connected by a single bond (biphenyl backbone). Each ring has an amino (-NH?) group at the 3 (meta) position relative to the inter-ring bond, making it a 3,3'-diaminobiphenyl.

Molecular Formula: C??H??N? (when considering its tetrahydrochloride form, commonly used in labs).

Key Applications

1. Chromogen in Staining:

   Widely used in immunohistochemistry (IHC) and enzyme-linked immunosorbent assays (ELISA).                                                    When oxidized by hydrogen peroxide (H?O?) in the presence of horseradish peroxidase (HRP), it produces an insoluble brown precipitate. This reaction helps visualize specific proteins or antigens in tissue samples or assays.

2. Electron Microscopy:

   Used to enhance contrast in samples by forming electron-dense reaction products.

Safety and Handling

Carcinogenicity: DAB is a derivative of benzidine, a known carcinogen. While DAB itself is less potent, it is still considered hazardous and potentially carcinogenic.

Precautions: Requires careful handling with gloves, lab coats, and proper ventilation to avoid inhalation or skin contact.

Regulatory Notes

Subject to strict safety regulations in laboratories. Alternatives (e.g., non-carcinogenic chromogens) are sometimes used to mitigate risks.

Summary

3,3'-Diaminobenzidine is a critical reagent in biological staining, enabling precise detection of biomolecules. Its meta-positioned amino groups facilitate specific oxidative reactions, making it invaluable in research and diagnostics despite its associated health risks.